Assay of uridine diphosphate glucuronosyltransferase by high-pressure liquid chromatography

Michio Matsui; Fusako Nagai

doi:10.1016/0003-2697(80)90435-2

Assay of uridine diphosphate glucuronosyltransferase by high-pressure liquid chromatography

Michio Matsui, Fusako Nagai

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A new and simple method for the determination of rat liver microsomal uridine diphosphate glucuronosyltransferase activity toward 4-nitrophenol, phenolphthalein, and testosterone has been developed with the use of high-pressure liquid chromatography (hplc). After incubation of the substrate with microsomal fractions in the presence of uridine diphosphate glucuronic acid, the reaction is stopped by heating, mixed with methanol, and centrifuged to give the supernatant, which is analyzed directly by hplc. The unreacted substrate as well as its glucuronide can be quantitated from the peak-height method. When the pure glucuronide is not available, the standard curve for the glucuronide is obtained from the enzymatically produced glucuronide.

Original language	English
Pages (from-to)	141-146
Number of pages	6
Journal	Analytical Biochemistry
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90435-2
Publication status	Published - 1980 Jun

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A new and simple method for the determination of rat liver microsomal uridine diphosphate glucuronosyltransferase activity toward 4-nitrophenol, phenolphthalein, and testosterone has been developed with the use of high-pressure liquid chromatography (hplc). After incubation of the substrate with microsomal fractions in the presence of uridine diphosphate glucuronic acid, the reaction is stopped by heating, mixed with methanol, and centrifuged to give the supernatant, which is analyzed directly by hplc. The unreacted substrate as well as its glucuronide can be quantitated from the peak-height method. When the pure glucuronide is not available, the standard curve for the glucuronide is obtained from the enzymatically produced glucuronide.",

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Assay of uridine diphosphate glucuronosyltransferase by high-pressure liquid chromatography

Abstract

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