TY - JOUR
T1 - Assessment of homozygosity levels in the mito-gynogenetic torafugu (Takifugu rubripes) by genome-wide SNP analyses
AU - Zhang, Hong
AU - Hirose, Yusuke
AU - Yoshino, Rintaro
AU - Kinoshita, Shigeharu
AU - Saito, Kazuyoshi
AU - Tan, Engkong
AU - Suzuki, Yutaka
AU - Shimizu, Atsushi
AU - Okano, Hideyuki
AU - Kudoh, Jun
AU - Watabe, Shugo
AU - Asakawa, Shuichi
PY - 2013/3/4
Y1 - 2013/3/4
N2 - The compact genome of torafugu (Takifugu rubripes) is a useful model for annotating the vertebrate genomes. However, the currently incomplete assembly of the torafugu genome has decreased its value for genetic and genomic analyses. This is partly due to the usage of naturally heterozygous diploid genome as a starting material. Therefore, we generated mito-gynogenetic torafugu using the eggs fertilized by UV-irradiated sperm, followed by cold shock treatment. After a preliminary confirmation of homozygosity by microsatellite genotyping in one mito-gynogenetic torafugu, genome-wide single nucleotide polymorphism (SNP) analyses were employed for an accurate assessment of whole-genome homozygosity levels. SNP genotyping results indicated that the torafugu obtained through mitotic gynogenesis induction was doubled haploid. We report the first instance of assessing homozygosity levels by genome-wide SNP analyses in mito-gynogenetic fishes.
AB - The compact genome of torafugu (Takifugu rubripes) is a useful model for annotating the vertebrate genomes. However, the currently incomplete assembly of the torafugu genome has decreased its value for genetic and genomic analyses. This is partly due to the usage of naturally heterozygous diploid genome as a starting material. Therefore, we generated mito-gynogenetic torafugu using the eggs fertilized by UV-irradiated sperm, followed by cold shock treatment. After a preliminary confirmation of homozygosity by microsatellite genotyping in one mito-gynogenetic torafugu, genome-wide single nucleotide polymorphism (SNP) analyses were employed for an accurate assessment of whole-genome homozygosity levels. SNP genotyping results indicated that the torafugu obtained through mitotic gynogenesis induction was doubled haploid. We report the first instance of assessing homozygosity levels by genome-wide SNP analyses in mito-gynogenetic fishes.
KW - Doubled haploid
KW - Homozygosity
KW - Mitotic gynogenesis
KW - Next-generation sequencing
KW - Single nucleotide polymorphism
KW - Torafugu (Takifugu rubripes)
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U2 - 10.1016/j.aquaculture.2012.12.003
DO - 10.1016/j.aquaculture.2012.12.003
M3 - Article
AN - SCOPUS:84872119842
SN - 0044-8486
VL - 380-383
SP - 114
EP - 119
JO - Aquaculture
JF - Aquaculture
ER -