A cDNA coding for human myeloperoxidase (MPO) was used as a probe to study MPO gene structure and to determine the chromosomal location of the gene in the human genome. Southern blot hybridization of restriction endonuclease digests of human DNA with the MPO cDNA probe showed that a single gene for human MPO was present in the human genome. Southern blot hybridization experiments with human-mouse cell hybrid DNAs containing various subsets of human chromosomes revealed that the human MPO gene is located on chromosome 17. This conclusion was supported by DNA spot-blot hybridization using flow-sorted human chromosomes.
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