TY - JOUR
T1 - AT-hook DNA-binding motif-containing protein one knockdown downregulates EWSFLI1 transcriptional activity in Ewing’s sarcoma cells
AU - Kitagawa, Takao
AU - Kobayashi, Daiki
AU - Baron, Byron
AU - Okita, Hajime
AU - Miyamoto, Tatsuo
AU - Takai, Rie
AU - Paudel, Durga
AU - Ohta, Tohru
AU - Asaoka, Yoichi
AU - Tokunaga, Masayuki
AU - Nakagawa, Koji
AU - Furutani-Seiki, Makoto
AU - Araki, Norie
AU - Kuramitsu, Yasuhiro
AU - Kobayashi, Masanobu
N1 - Funding Information:
This research was supported by Grant-in-Aid for Scientific Research (C) (Grant number: 18K07199) from the Japan Society for the Promotion of Science (to TK) and the Specific Research Grants (Grant number: 2019081196) from the Takeda Science Foundation (to MFS).
Publisher Copyright:
© 2022 Kitagawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2022/10
Y1 - 2022/10
N2 - Ewing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing’s sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1.
AB - Ewing’s sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing’s sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1.
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U2 - 10.1371/journal.pone.0269077
DO - 10.1371/journal.pone.0269077
M3 - Article
C2 - 36194562
AN - SCOPUS:85139561472
SN - 1932-6203
VL - 17
JO - PloS one
JF - PloS one
IS - 10 October
M1 - e0269077
ER -