Atrial natriuretic polypeptide as a novel antigrowth factor of endothelial cells

Hiroshi Itoh, Richard E. Pratt, Minoru Ohno, Victor J. Dzau

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)


The migration and proliferation of endothelial cells play a pivotal role in various vascular diseases. We have previously reported that atrial natriuretic polypeptide (ANP) exerts an antigrowth effect on vascular smooth muscle cells via the guanylate cyclase-coupled mechanism. Because the endothelial cells are known to possess a large number of guanylate cyclase-coupled ANP receptors, we examined the action of ANP on the growth of endothelial cells. ANP (10−7 and 10−6 M) significantly attenuated serum-stimulated DNA synthesis of cultured bovine aortic endothelial cells with concomitant reduction of the increase in cell number. A ring-deleted analogue of ANP exerted less prominent antiproliferative action, and 8-bromo cyclic GMP (cGMP) mimicked the action of ANP, suggesting the involvement of cGMP cascade in the endothelial growth. Moreover, the proliferative action of exogenously administered basic fibroblast growth factor on endothelial cells was significantly attenuated by the simultaneously administered 8-bromo cGMP. Taken together, the present results demonstrate a potential novel role of ANP in the regulation of endothelial cell growth, which is implicated in angiogenesis or reendothelialization.

Original languageEnglish
Pages (from-to)758-761
Number of pages4
Issue number6
Publication statusPublished - 1992 Jun
Externally publishedYes


  • Atherosclerosis
  • Atrial natriuretic peptides
  • Cell division
  • Cyclic GMP
  • Endothelial growth factors
  • Endothelium
  • Fibroblast growth factor
  • Neovascularization

ASJC Scopus subject areas

  • Internal Medicine


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