Atrial natriuretic polypeptide in bovine adrenal medulla

Masashi Mukoyama, Kazuwa Nakao, Narito Morii, Shozo Shiono, Hiroshi Itoh, Akira Sugawara, Takayuki Yamada, Yoshihiko Saito, Hiroshi Arai, Hiroo Mura

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Two radioimmunoassays for α-human atrial natriuretic polypeptide (a-hANP) with different specificities were used to study the tissue level and the nature of α-hANP-like immunoreactivity in the bovine adrenal gland. A considerable amount of α-hANP-like immunoreactivity was detected in the adrenal medulla (90.8 ± 21.1 and 90.0 ± 23.1 ng/g with the two radioimmunoassays), while no detectable amount (<1.0 μg) was present in the cortex. Gel permeation chromatographic analysis showed that ANP in the medulla is composed of two components of α-hANP-like immunoreactivity with high and low molecular weights in the approximate ratio of 2:1, eluting at the elution positions of y-hANP and α-hANP, respectively. Reverse-phase high performance liquid chromatographic analysis revealed that α-hANP-like immunoreactivity with a low molecular weight in the medulla consists of two major components, which comigrate with synthetic α-hANP(5-28) and ahANP. When cultured bovine adrenal chrom'affin cells were incubated in the presence of nicotine (10-5 M), α-hANP-like immunoreactivity was released into the medium concomitantly with catecholamines from chromaffin cells. These findings indicate that a discrete ANP system is present in the adrenal medulla and that ANP is cosecreted with catecholamines from chromaffin cells, suggesting the possible involvement of ANP in the adrenomedullary function.

Original languageEnglish
Pages (from-to)692-696
Number of pages5
Issue number6
Publication statusPublished - 1988 Jun
Externally publishedYes


  • Adrenomedullary function
  • Atrial natriuretic factor
  • Catecholamine
  • Chromaffin cell
  • Nicotine
  • Radioimmunoassay

ASJC Scopus subject areas

  • Internal Medicine


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