TY - JOUR
T1 - Atrial natriuretic polypeptide in spinal cord and autonomic ganglia
AU - Morii, Narito
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Shiono, Shozo
AU - Yamada, Takayuki
AU - Sugawara, Akira
AU - Saito, Yoshihiko
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Sakamoto, Makoto
AU - Imura, Hiroo
N1 - Funding Information:
We thank Mrs. H. Tabata and Miss A. Furu for assistance. This work was supported in part by research
PY - 1987/5/29
Y1 - 1987/5/29
N2 - Using a radioimmunoassay for α-rat atrial natriuretic polypeptide (α-rANP), tissue levels of α-rANP-like immunoreactivity (-LI) in the rat spinal cord and autonomic ganglia were investigated. The α-rANP-LI level was higher in the more caudal parts of the spinal cord and the highest in the sacral spinal cord. α-rANP-LI was also detected in the superior cervical and coeliac ganglia. Gel permeation chromatographic analysis showed that the major peak of α-rANP-LI in the spinal cord was a low molecular weight form co-eluted with synthetic α-rANP. Reverse-phase high performance liquid chromatographic analysis revealed that α-rANP-LI with a low molecular weight in the spinal cord consisted of several components, two major components of which co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), whereas little immunoreactivity was eluted at the position of α-rANP. These findings suggest the involvement of ANP in the function of the spinal cord and autonomic nervous system.
AB - Using a radioimmunoassay for α-rat atrial natriuretic polypeptide (α-rANP), tissue levels of α-rANP-like immunoreactivity (-LI) in the rat spinal cord and autonomic ganglia were investigated. The α-rANP-LI level was higher in the more caudal parts of the spinal cord and the highest in the sacral spinal cord. α-rANP-LI was also detected in the superior cervical and coeliac ganglia. Gel permeation chromatographic analysis showed that the major peak of α-rANP-LI in the spinal cord was a low molecular weight form co-eluted with synthetic α-rANP. Reverse-phase high performance liquid chromatographic analysis revealed that α-rANP-LI with a low molecular weight in the spinal cord consisted of several components, two major components of which co-migrated with synthetic α-rANP(4-28) and α-rANP(5-28), whereas little immunoreactivity was eluted at the position of α-rANP. These findings suggest the involvement of ANP in the function of the spinal cord and autonomic nervous system.
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U2 - 10.1016/0006-291X(87)91306-4
DO - 10.1016/0006-291X(87)91306-4
M3 - Article
C2 - 2954548
AN - SCOPUS:0023228994
SN - 0006-291X
VL - 145
SP - 196
EP - 203
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -