Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells

Sho Yoshimatsu, Mayutaka Nakajima, Iki Sonn, Rie Natsume, Kenji Sakimura, Ena Nakatsukasa, Toshikuni Sasaoka, Mari Nakamura, Takashi Serizawa, Tsukika Sato, Erika Sasaki, Hongkui Deng, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review


Extended pluripotent stem cells (EPSCs) derived from mice and humans showed an enhanced potential for chimeric formation. By exploiting transcriptomic approaches, we assessed the differences in gene expression profile between extended EPSCs derived from mice and humans, and those newly derived from the common marmoset (marmoset; Callithrix jacchus). Although the marmoset EPSC-like cells displayed a unique colony morphology distinct from murine and human EPSCs, they displayed a pluripotent state akin to embryonic stem cells (ESCs), as confirmed by gene expression and immunocytochemical analyses of pluripotency markers and three-germ-layer differentiation assay. Importantly, the marmoset EPSC-like cells showed interspecies chimeric contribution to mouse embryos, such as E6.5 blastocysts in vitro and E6.5 epiblasts in vivo in mouse development. Also, we discovered that the perturbation of gene expression of the marmoset EPSC-like cells from the original ESCs resembled that of human EPSCs. Taken together, our multiple analyses evaluated the efficacy of the method for the derivation of marmoset EPSCs.

Original languageEnglish
Pages (from-to)156-169
Number of pages14
JournalGenes to Cells
Issue number2
Publication statusPublished - 2023 Feb


  • RNA-seq
  • common marmoset
  • embryonic stem cells
  • extended
  • induced
  • nonhuman primate
  • pluripotent stem cells
  • pluripotent stem cells
  • pluripotent stem cells
  • transcriptomics

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


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