@article{9114551512084aa4afa606df483d39fb,
title = "Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells",
abstract = "Extended pluripotent stem cells (EPSCs) derived from mice and humans showed an enhanced potential for chimeric formation. By exploiting transcriptomic approaches, we assessed the differences in gene expression profile between extended EPSCs derived from mice and humans, and those newly derived from the common marmoset (marmoset; Callithrix jacchus). Although the marmoset EPSC-like cells displayed a unique colony morphology distinct from murine and human EPSCs, they displayed a pluripotent state akin to embryonic stem cells (ESCs), as confirmed by gene expression and immunocytochemical analyses of pluripotency markers and three-germ-layer differentiation assay. Importantly, the marmoset EPSC-like cells showed interspecies chimeric contribution to mouse embryos, such as E6.5 blastocysts in vitro and E6.5 epiblasts in vivo in mouse development. Also, we discovered that the perturbation of gene expression of the marmoset EPSC-like cells from the original ESCs resembled that of human EPSCs. Taken together, our multiple analyses evaluated the efficacy of the method for the derivation of marmoset EPSCs.",
keywords = "RNA-seq, common marmoset, embryonic stem cells, extended, induced, nonhuman primate, pluripotent stem cells, pluripotent stem cells, pluripotent stem cells, transcriptomics",
author = "Sho Yoshimatsu and Mayutaka Nakajima and Iki Sonn and Rie Natsume and Kenji Sakimura and Ena Nakatsukasa and Toshikuni Sasaoka and Mari Nakamura and Takashi Serizawa and Tsukika Sato and Erika Sasaki and Hongkui Deng and Hideyuki Okano",
note = "Funding Information: We greatly thank Drs. Takashi Sasaki (Keio University) and Tsukasa Sanosaka (Keio University) for kind support and technical advice for RNA‐seq analysis, Ms. Kanae Ohtsu (Keio University) for technical supports for this study. We also thank all the laboratory members of H.O. for their encouragement and generous supports for this study. Portions of this study were the result of the “Construction of System for Spread of Primate Model Animals”, which was performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the MEXT and the AMED (Grant No. JP20dm0207001, to H.O.), and Scientific Research in Innovative Areas, which is the MEXT Grant‐in‐Aid Project FY2014‐2018: “Brain Protein Aging and Dementia Control” (Grant No. 26117007, to H.O.). S.Y. was financially supported by RIKEN Junior Research Associate Program. This research was also supported by internal budgets from Keio University, Osaka University, including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.). Funding Information: Japan Agency for Medical Research and Development, Grant/Award Number: JP20dm0207001; Ministry of Education, Culture, Sports, Science and Technology, Grant/Award Number: 26117007; RIKEN; Keio University; Osaka University Funding information Publisher Copyright: {\textcopyright} 2022 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.",
year = "2023",
month = feb,
doi = "10.1111/gtc.13000",
language = "English",
volume = "28",
pages = "156--169",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "2",
}