Azaelectrocyclization on cell surface: Convenient and general approach to chemical biology research

Akihiro Ogura, Katsunori Tanaka

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Convenient and general method for live cell surface labeling was established. Azide-containing fluorescences were initially conjugated with the unsaturated ester aldehyde containing the dibenzocyclooctyne (DIBO), by the strain-promoted click reaction. The resulting probes were then treated with the live cells in one-pot process, efficiently labeling the surface lysines by azaelectrocyclization under the mild conditions.

Original languageEnglish
Pages (from-to)4518-4521
Number of pages4
JournalTetrahedron
Volume71
Issue number26-27
DOIs
Publication statusPublished - 2015 May 30
Externally publishedYes

Keywords

  • Azaelectrocyclization
  • Fluorescence
  • Labeling
  • Live cell
  • Lysine

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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