B7-H3 ligand expression by primary breast cancer and associated with regional nodal metastasis

Takaaki Arigami, Norihiko Narita, Ryuichi Mizuno, Linhda Nguyen, Xing Ye, Alice Chung, Armando E. Giuliano, Dave S.B. Hoon

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)


Objective: B7 ligand family members have been shown to be important immunoregulatory factors in host tumor immune responses. We hypothesized that B7-H3, a coinhibitory factor, is expressed by primary breast cancer cells and associated with metastasis to regional tumor-draining lymph nodes. EXPERIMENTAL DESIGN:: American Joint Committee on Cancer stage I to III primary breast cancers (n = 82) and normal breast specimens (n = 17) were assessed for B7-H3 expression using paraffin-embedded archival tissues. B7-H3 expression by breast cancer cells was assessed by a quantitative real-time reverse transcription-polymerase chain reaction, and B7-H3 protein expression was evaluated using immunohistochemistry. Results: B7-H3 mRNA expression was detected in 32 of 82 (39%) primary breast tumors but not in normal breast tissues (P = 0.0029). B7-H3 expression in primary tumors significantly correlated with increasing tumor size, American Joint Committee on Cancer stage, and lymphovascular invasion (P < 0.0001, P < 0.0001, P = 0.0071). B7-H3 expression was highly correlated to sentinel lymph node and overall number of lymph nodes with metastasis P = 0.003, and P = 0.004, respectively). In a multivariate analysis, B7-H3 mRNA expression of the primary tumor significantly predicted metastasis to regional lymph nodes (P = 0.021, and P = 0.023, respectively). Antibody staining analysis of paraffin-embedded archival tissue breast tumors and flow cytometry of breast cancer cell lines demonstrated B7-H3 protein expression. Conclusions: B7-H3 protein expressed by primary breast cancer cells is a tumor progression factor and is associated with extent of regional nodal metastasis.

Original languageEnglish
Pages (from-to)1044-1051
Number of pages8
JournalAnnals of surgery
Issue number6
Publication statusPublished - 2010 Dec
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'B7-H3 ligand expression by primary breast cancer and associated with regional nodal metastasis'. Together they form a unique fingerprint.

Cite this