Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

Ryo Iwaoka; Takashi Nagata; Kengo Tsuda; Takao Imai; Hideyuki Okano; Naohiro Kobayashi; Masato Katahira

doi:10.1007/s12104-017-9760-9

Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

Ryo Iwaoka, Takashi Nagata, Kengo Tsuda, Takao Imai, Hideyuki Okano, Naohiro Kobayashi, Masato Katahira

Department of Physiology

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the ¹H, ¹⁵N, and ¹³C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.

Original language	English
Pages (from-to)	265-268
Number of pages	4
Journal	Biomolecular NMR Assignments
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/s12104-017-9760-9
Publication status	Published - 2017 Oct 1

Keywords

Cancer development
Cell fate determination
Musashi
RBD
RNA-binding protein
RRM

ASJC Scopus subject areas

Structural Biology
Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s12104-017-9760-9

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title = "Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA",

abstract = "Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.",

keywords = "Cancer development, Cell fate determination, Musashi, RBD, RNA-binding protein, RRM",

author = "Ryo Iwaoka and Takashi Nagata and Kengo Tsuda and Takao Imai and Hideyuki Okano and Naohiro Kobayashi and Masato Katahira",

note = "Funding Information: Acknowledgements This work was supported by Japan Society for the Promotion of Science KAKENHI to M.K. (#15H01256, #16H00833, and #16K14678) and to T.N. (#15H01634 and #26440026). The authors would like to acknowledge Dr. Mikako Shi-rouzu for fruitful discussion. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media B.V.",

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month = oct,

day = "1",

doi = "10.1007/s12104-017-9760-9",

language = "English",

volume = "11",

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journal = "Biomolecular NMR Assignments",

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T1 - Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

AU - Iwaoka, Ryo

AU - Nagata, Takashi

AU - Tsuda, Kengo

AU - Imai, Takao

AU - Okano, Hideyuki

AU - Kobayashi, Naohiro

AU - Katahira, Masato

N1 - Funding Information: Acknowledgements This work was supported by Japan Society for the Promotion of Science KAKENHI to M.K. (#15H01256, #16H00833, and #16K14678) and to T.N. (#15H01634 and #26440026). The authors would like to acknowledge Dr. Mikako Shi-rouzu for fruitful discussion. Publisher Copyright: © 2017, Springer Science+Business Media B.V.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.

AB - Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the 1H, 15N, and 13C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.

KW - Cancer development

KW - Cell fate determination

KW - Musashi

KW - RBD

KW - RNA-binding protein

KW - RRM

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UR - http://www.scopus.com/inward/citedby.url?scp=85028574334&partnerID=8YFLogxK

U2 - 10.1007/s12104-017-9760-9

DO - 10.1007/s12104-017-9760-9

M3 - Article

C2 - 28808919

AN - SCOPUS:85028574334

SN - 1874-2718

VL - 11

SP - 265

EP - 268

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 2

ER -

Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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