Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states

Shunsuke Igarashi; Masanori Osawa; Shin Ichiro Ozawa; Ichio Shimada

doi:10.1007/s12104-009-9200-6

Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states

Shunsuke Igarashi, Masanori Osawa, Shin Ichiro Ozawa, Ichio Shimada

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

HisJ is a histidine binding subunit of the histidine permease, which exists in the outer membrane of Gram-negative bacteria. In order to incorporate the periplasmic histidine into the cell, HisJ captures histidine in the periplasm, and transfers the histidine to the transmembrane complex of histidine permease that is an ABC transporter. We established the backbone resonance assignments of ¹H/¹³C/¹⁵N-labeled HisJ from Escherichia coli, in the histidine-bound and unbound states.

Original language	English
Pages (from-to)	17-20
Number of pages	4
Journal	Biomolecular NMR Assignments
Volume	4
Issue number	1
DOIs	https://doi.org/10.1007/s12104-009-9200-6
Publication status	Published - 2010 Apr
Externally published	Yes

Keywords

ABC transporter
Apo HisJ
Histidine
Histidine permease
Holo HisJ

ASJC Scopus subject areas

Structural Biology
Biochemistry

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10.1007/s12104-009-9200-6

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title = "Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states",

abstract = "HisJ is a histidine binding subunit of the histidine permease, which exists in the outer membrane of Gram-negative bacteria. In order to incorporate the periplasmic histidine into the cell, HisJ captures histidine in the periplasm, and transfers the histidine to the transmembrane complex of histidine permease that is an ABC transporter. We established the backbone resonance assignments of 1H/13C/15N-labeled HisJ from Escherichia coli, in the histidine-bound and unbound states.",

keywords = "ABC transporter, Apo HisJ, Histidine, Histidine permease, Holo HisJ",

author = "Shunsuke Igarashi and Masanori Osawa and Ozawa, {Shin Ichiro} and Ichio Shimada",

note = "Funding Information: Acknowledgments This work was supported in part by grants from the Japan New Energy and Industrial Technology Development Organization (NEDO) and the Ministry of Economy, Trade, and Industry (METI) (to I.S.), a Grant-in-Aid for Scientific Research on Priority Areas from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (to M.O. and I.S.), and a grant from Takeda Science Foundation (to M.O.).",

year = "2010",

month = apr,

doi = "10.1007/s12104-009-9200-6",

language = "English",

volume = "4",

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journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Netherlands",

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T1 - Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states

AU - Igarashi, Shunsuke

AU - Osawa, Masanori

AU - Ozawa, Shin Ichiro

AU - Shimada, Ichio

N1 - Funding Information: Acknowledgments This work was supported in part by grants from the Japan New Energy and Industrial Technology Development Organization (NEDO) and the Ministry of Economy, Trade, and Industry (METI) (to I.S.), a Grant-in-Aid for Scientific Research on Priority Areas from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (to M.O. and I.S.), and a grant from Takeda Science Foundation (to M.O.).

PY - 2010/4

Y1 - 2010/4

N2 - HisJ is a histidine binding subunit of the histidine permease, which exists in the outer membrane of Gram-negative bacteria. In order to incorporate the periplasmic histidine into the cell, HisJ captures histidine in the periplasm, and transfers the histidine to the transmembrane complex of histidine permease that is an ABC transporter. We established the backbone resonance assignments of 1H/13C/15N-labeled HisJ from Escherichia coli, in the histidine-bound and unbound states.

AB - HisJ is a histidine binding subunit of the histidine permease, which exists in the outer membrane of Gram-negative bacteria. In order to incorporate the periplasmic histidine into the cell, HisJ captures histidine in the periplasm, and transfers the histidine to the transmembrane complex of histidine permease that is an ABC transporter. We established the backbone resonance assignments of 1H/13C/15N-labeled HisJ from Escherichia coli, in the histidine-bound and unbound states.

KW - ABC transporter

KW - Apo HisJ

KW - Histidine

KW - Histidine permease

KW - Holo HisJ

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Backbone resonance assignments for the ligand binding subunit of the histidine permease complex (HisJ) from Escherichia coli, under histidine-bound and unbound states

Abstract

Keywords

ASJC Scopus subject areas

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