TY - JOUR
T1 - Base liberation from nucleotides by superoxide and intramolecular enhancement effect of phosphate group
AU - Yamane, Hiroshi
AU - Yada, Nobuhisa
AU - Katori, Etsu
AU - Mashino, Tadahiko
AU - Nagano, Tetsuo
AU - Hirobe, Masaaki
N1 - Funding Information:
'This work was supported by a research grant from the Ministry of Education, Science and Culture of Japan. 2To whom correspondence should be addressed. Abbreviations: 02-, superoxide; CHO, Chinese hamster ovary; SOD, suoeroxide dismutase: 5'-AMP. adenosine-5'-monophosphate; 5'-GMP, guanosine-5'-monophosphate; 5'-CMP, cytidine:5'-monophosphate; 5'-UMP, uridine-5'-monophosphate; 3'-AMP, adenosine-3'-monophosphate; 3'-GMP, guanosine-3'-monophosphate; 3'-CMP, cytidine-3'-monophosphate; 3'-UMP, uridine-3'-monophosphate; A, adenosine; G, guanosine; C, cytidine; U, uridine; 5'-dAMP, 2'-deoxyadenosine-5'-monophosphate; 5'-dGMP, 2'-deoxyguanosine-5'-monophosphate; 5'-dGMP, 2'-deoxyguanosine-5'-monophosphate; dCMP, 2'-deoxycytidine-5'-monophosphate; 5'-dTMP, deoxythymidine-5'-monophosphate; 2'-deoxyadenosine; deoxyguanosine; dC, 2'-deoxycytidine; dT, 2'-deoxythymidine; AMP, adenosine-2'-monophosphate; 3',5'-CAMP, adenosine-3',5'-cyclic monophosphate; ADP, adenosine-5'-diphosphate; DMSO, dimethyl sulfoxide.
PY - 1987/2/13
Y1 - 1987/2/13
N2 - The reaction of nucleotides with superoxide gave the corresponding nucleobases in good yield. The base-release reaction of nucleotides due to superoxide was examined and compared to that of nucleosides. Hence, the phosphate moiety greatly enhances the yields, in particular those of adenine from adenosine monophosphates. Superoxide in combination with the phosphate moiety has been revealed to be more active than superoxide alone. The phosphate peroxy radical, generated in situ from superoxide and phosphate, seems to be the species responsible for the formation of the free bases.
AB - The reaction of nucleotides with superoxide gave the corresponding nucleobases in good yield. The base-release reaction of nucleotides due to superoxide was examined and compared to that of nucleosides. Hence, the phosphate moiety greatly enhances the yields, in particular those of adenine from adenosine monophosphates. Superoxide in combination with the phosphate moiety has been revealed to be more active than superoxide alone. The phosphate peroxy radical, generated in situ from superoxide and phosphate, seems to be the species responsible for the formation of the free bases.
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U2 - 10.1016/0006-291X(87)91529-4
DO - 10.1016/0006-291X(87)91529-4
M3 - Article
C2 - 3030294
AN - SCOPUS:0023095601
SN - 0006-291X
VL - 142
SP - 1104
EP - 1110
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -