Better method for detection of CD30: Immunohistochemistry or flow cytometry?

Morihiro Higashi; Jun Kikuchi; Chiaki Murakami; Natsuko Takayanagi; Shuji Momose; Masahiro Kizaki; Jun Ichi Tamaru

doi:10.3960/jslrt.21019

Better method for detection of CD30: Immunohistochemistry or flow cytometry?

Morihiro Higashi
, Jun Kikuchi
, Chiaki Murakami
, Natsuko Takayanagi
, Shuji Momose
, Masahiro Kizaki
, Jun Ichi Tamaru

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.

Original language	English
Pages (from-to)	221-223
Number of pages	3
Journal	Journal of Clinical and Experimental Hematopathology
Volume	61
Issue number	4
DOIs	https://doi.org/10.3960/jslrt.21019
Publication status	Published - 2021
Externally published	Yes

Keywords

CD30
T-cell lymphoma
diffuse large B-cell lymphoma
flow cytometry
immunohistochemistry

ASJC Scopus subject areas

General Medicine

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10.3960/jslrt.21019

Cite this

@article{a4b9a246ff7c4d3c8b1f48e136235f10,

title = "Better method for detection of CD30: Immunohistochemistry or flow cytometry?",

abstract = "We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.",

keywords = "CD30, T-cell lymphoma, diffuse large B-cell lymphoma, flow cytometry, immunohistochemistry",

author = "Morihiro Higashi and Jun Kikuchi and Chiaki Murakami and Natsuko Takayanagi and Shuji Momose and Masahiro Kizaki and Tamaru, \{Jun Ichi\}",

note = "Publisher Copyright: {\textcopyright} 2021 The Japanese Society for Lymphoreticular Tissue Research.",

year = "2021",

doi = "10.3960/jslrt.21019",

language = "English",

volume = "61",

pages = "221--223",

journal = "Journal of Clinical and Experimental Hematopathology",

issn = "1346-4280",

publisher = "Nihon Rinpa Monaikei Gakkai",

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TY - JOUR

T1 - Better method for detection of CD30

T2 - Immunohistochemistry or flow cytometry?

AU - Higashi, Morihiro

AU - Kikuchi, Jun

AU - Murakami, Chiaki

AU - Takayanagi, Natsuko

AU - Momose, Shuji

AU - Kizaki, Masahiro

AU - Tamaru, Jun Ichi

PY - 2021

Y1 - 2021

N2 - We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.

AB - We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.

KW - CD30

KW - T-cell lymphoma

KW - diffuse large B-cell lymphoma

KW - flow cytometry

KW - immunohistochemistry

UR - https://www.scopus.com/pages/publications/85147393409

UR - https://www.scopus.com/pages/publications/85147393409#tab=citedBy

U2 - 10.3960/jslrt.21019

DO - 10.3960/jslrt.21019

M3 - Article

AN - SCOPUS:85147393409

SN - 1346-4280

VL - 61

SP - 221

EP - 223

JO - Journal of Clinical and Experimental Hematopathology

JF - Journal of Clinical and Experimental Hematopathology

IS - 4

ER -

Better method for detection of CD30: Immunohistochemistry or flow cytometry?

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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