Biodegradable polycaprolactone nanofibres with β-chitosan and calcium carbonate produce a hemostatic effect

We newly developed high-performance blood coagulation nanofibres using Polycaprolactone (PCL) including Calcium carbonate (CaCO₃) and β-chitosan. The most important feature for a wound dressing is that it is harmless to the human body. Here, we fabricated the nanofibre using all human-safe materials. We used a PCL nanofibre mat as a substrate and then synthesized artificial CaCO₃ from sodium carbonate and calcium chloride. The CaCO₃ was then added to the fibre solution to create PCL/CaCO₃ nanofibres. We coated PCL and PCL/CaCO₃ nanofibres with β-chitosan as a hemostatic material via a spray method. For the more uniform coating, we used ultrasonic spray coating method and then compared the blood coagulation abilities of the PCL and PCL/CaCO₃ nanofibres. We found that the PCL/CaCO₃ nanofibres sprayed with β-chitosan had a greater effect on blood coagulation than the PCL nanofibres. In the result of animal experiment, β-chitosan have a key role in changing of surface wettability from hydrophobic to hydrophilic. Moreover, this is contributed to enhance blood coagulability. PCL/CaCO₃ nanofibres sprayed with β-chitosan therefore offer promise in medical applications.