Biofabrication of living vessel structures integrated with fluid perfusion

Shintaroh Iwanaga, Shigenori Miura, Hiroaki Onoe, Teru Okitsu, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper describes a biofabrication method of vessel-like structure with our gel fiber technology. Alginate was modified with gelatin to promote cell adhesion and proliferation, and treated with fibronectin (FN). Vascular endothelial cells (ECs) adhered and proliferated well on the modified alginate fiber. After embedding EC-coated fiber into extracellular matrix (ECM), ECs were transferred to ECM from the fiber, and then the fiber was easily removed with alginate lyase. Fluoresceinlabeled materials were flushed into the lumen, and it was observed small-sized molecules were leaked out to ECM. This approach would be applicable for the rapid biofabrication of functional luminal tissues.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1723-1725
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • Endothelial cell
  • Hydrogel fiber
  • Tissue engineering
  • Vessel structure

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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