@article{73bd439fda0048638daad5879ebc41ab,
title = "Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus",
abstract = "A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of ∼12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be ∼30 kDa and ∼33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10 mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.",
keywords = "Boletaceae, Boletus venenatus, Bolevenine, Mushroom poisoning, Toxic protein, Trimeric protein",
author = "Masanori Matsuura and Mina Yamada and Yoko Saikawa and Kazuo Miyairi and Toshikatsu Okuno and Katsuhiro Konno and Jun'ichi Uenishi and Kimiko Hashimoto and Masaya Nakata",
note = "Funding Information: We are grateful to Mr. Takashi Suda and Mr. Toshiro Masai for collecting the mushrooms. We thank Dr. Shuichi Matsumura (Keio University) for technical assistance with SDS–PAGE. We are indebted to Mr. Takashi Hamada (Kyoto Pharmaceutical University) and Mr. Jyunji Ichita (Aomori Advanced Industrial Technology Center) for MALDI-TOFMS measurements. This research was partially supported by a Grant-in-Aid from the 21st Century COE program “KEIO Life Conjugate Chemistry” (M.M., Y.S.), Frontier Research Program (J.U., K.H.), and the 21st Century COE Program “Development of Drug Discovery Frontier Integrated from Tradition to Proteome” (J.U., K.H.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT).",
year = "2007",
month = mar,
doi = "10.1016/j.phytochem.2006.11.037",
language = "English",
volume = "68",
pages = "893--898",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "6",
}