Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche

Yoshiaki Kubota, Keiyo Takubo, Toshio Suda

Research output: Contribution to journalArticlepeer-review

119 Citations (Scopus)


In response to changing signals, quiescent hematopoietic stem cells (HSCs) can be induced to an activated cycling state and provide multi-lineage hematopoietic cells to the whole body via blood vessels. However, the precise localization of quiescent HSCs in bone marrow microenvironment is not fully characterized. Here, we performed whole-mount immunostaining of bone marrow and found that BrdU label-retaining cells (LRCs) definitively reside in the sinusoidal hypoxic zone distant from the "vascular niche". Although LRCs expressed very low level of a well-known HSC marker, c-kit in normal circumstances, myeloablation by 5-FU treatment caused LRCs to abundantly express c-kit and proliferate actively. These results demonstrate that bone marrow LRCs reside in the sinusoidal hypoxic niche, and function as a regenerative cell pool of HSCs.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 2008 Feb 8


  • Hematopoietic stem cell
  • Hypoxia
  • Hypoxic niche
  • Label-retaining cell
  • Osteoblastic niche
  • Quiescence
  • Sinusoid
  • Sinusoidal niche
  • Vascular niche
  • c-kit

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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