Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki, Kenji Tsuge

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)


We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

Original languageEnglish
Pages (from-to)41-43
Number of pages3
JournalNature Methods
Issue number1
Publication statusPublished - 2008 Jan

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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