Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya; Kyoko Fujita; Azusa Kuroki; Kenji Tsuge

doi:10.1038/nmeth1143

Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki, Kenji Tsuge

Faculty of Environment and Information Studies

Research output: Contribution to journal › Article › peer-review

126 Citations (Scopus)

Abstract

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

Original language	English
Pages (from-to)	41-43
Number of pages	3
Journal	Nature Methods
Volume	5
Issue number	1
DOIs	https://doi.org/10.1038/nmeth1143
Publication status	Published - 2008 Jan

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth1143

Cite this

@article{15f406d38e904ea8b32c2ba5734c8ad8,

title = "Bottom-up genome assembly using the Bacillus subtilis genome vector",

abstract = "We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.",

author = "Mitsuhiro Itaya and Kyoko Fujita and Azusa Kuroki and Kenji Tsuge",

year = "2008",

month = jan,

doi = "10.1038/nmeth1143",

language = "English",

volume = "5",

pages = "41--43",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Bottom-up genome assembly using the Bacillus subtilis genome vector

AU - Itaya, Mitsuhiro

AU - Fujita, Kyoko

AU - Kuroki, Azusa

AU - Tsuge, Kenji

PY - 2008/1

Y1 - 2008/1

N2 - We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

AB - We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

UR - http://www.scopus.com/inward/record.url?scp=37749014043&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37749014043&partnerID=8YFLogxK

U2 - 10.1038/nmeth1143

DO - 10.1038/nmeth1143

M3 - Article

C2 - 18066072

AN - SCOPUS:37749014043

SN - 1548-7091

VL - 5

SP - 41

EP - 43

JO - Nature Methods

JF - Nature Methods

IS - 1

ER -

Bottom-up genome assembly using the Bacillus subtilis genome vector

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this