Capillary-based centrifugal microfluidic device for size-controllable formation of monodisperse microdroplets

Masamune Morita, Hitoyoshi Yamashita, Masayuki Hayakawa, Hiroaki Onoe, Masahiro Takinoue

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using a capillarybased centrifugal microfluidic device. W/O microdroplets have recently been used in powerful methods that enable miniaturized chemical experiments. Therefore, developing a versatile method to yield monodisperse W/O microdroplets is needed. We have developed a method for generating monodisperse W/O microdroplets based on a capillary-based centrifugal axisymmetric co-flowing microfluidic device. We succeeded in controlling the size of microdroplets by adjusting the capillary orifice. Our method requires equipment that is easier-to-use than with other microfluidic techniques, requires only a small volume (0.1-1 μl) of sample solution for encapsulation, and enables the production of hundreds of thousands number of W/O microdroplets per second. We expect this method will assist biological studies that require precious biological samples by conserving the volume of the samples for rapid quantitative analysis biochemical and biological studies.

Original languageEnglish
Article numbere53860
JournalJournal of Visualized Experiments
Issue number108
Publication statusPublished - 2016 Feb 22


  • Axisymmetricco-flowing
  • Capillary-based centrifugal microfluidic device
  • Droplet microfluidics
  • Engineering
  • Issue 108
  • Water-in-oil microdroplets

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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