TY - JOUR
T1 - Carbohydrate recognition by pentadecapeptide ligands for a series of sialylated oligosaccharides
AU - Matsubara, Teruhiko
AU - Onishi, Ai
AU - Sato, Toshinori
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research B (14380411, TS) and a Grant-in-Aid for Young Scientists B (17750166, TM) from the Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government.
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Sialyloligosaccharides of glycoproteins and glycosphingolipids play important roles in biological events on cell membranes. GT1b is a ganglioside having a trisialyloligosaccharide and is a receptor for tetanus toxin. In the present study, pentadecapeptide ligands for GT1b were obtained by phage display selection from a random peptide library with the use of a GT1b monolayer. The artificial pentadecapeptides had high affinity for GT1b which tended to increase depending on the number of sialic acids in sialyloligosaccharides. Arg, Ser, and hydrophobic amino acids were found in a consensus motif and may contribute to carbohydrate recognition. The consensus motif of the GT1b-binding peptides was different from that of GM1-, GM2-, GM3-, or GD1a-binding peptides. Peptide ligands for GT1b should be investigated for trisialyloligosaccharide functions and the development of therapeutic agents against trisialyloligosaccharide- related diseases.
AB - Sialyloligosaccharides of glycoproteins and glycosphingolipids play important roles in biological events on cell membranes. GT1b is a ganglioside having a trisialyloligosaccharide and is a receptor for tetanus toxin. In the present study, pentadecapeptide ligands for GT1b were obtained by phage display selection from a random peptide library with the use of a GT1b monolayer. The artificial pentadecapeptides had high affinity for GT1b which tended to increase depending on the number of sialic acids in sialyloligosaccharides. Arg, Ser, and hydrophobic amino acids were found in a consensus motif and may contribute to carbohydrate recognition. The consensus motif of the GT1b-binding peptides was different from that of GM1-, GM2-, GM3-, or GD1a-binding peptides. Peptide ligands for GT1b should be investigated for trisialyloligosaccharide functions and the development of therapeutic agents against trisialyloligosaccharide- related diseases.
KW - Ganglioside GT1b
KW - Phage display
KW - Random peptide library
KW - Sialic acid
KW - Sialyloligosaccharide
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U2 - 10.1016/j.bmc.2012.08.025
DO - 10.1016/j.bmc.2012.08.025
M3 - Article
C2 - 23000297
AN - SCOPUS:84867579033
SN - 0968-0896
VL - 20
SP - 6452
EP - 6458
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 21
ER -