CD200-expressing human basal cell carcinoma cells initiate tumor growth

Chantal S. Colmont, Antisar BenKetah, Simon H. Reed, Nga V. Hawk, William G. Telford, Manabu Ohyama, Mark C. Udey, Carole L. Yee, Jonathan C. Vogel, Girish K. Patel

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Smoothened antagonists directly target the genetic basis of human basal cell carcinoma (BCC), the most common of all cancers. These drugs inhibit BCC growth, but they are not curative. Although BCC cells are monomorphic, immunofluorescence microscopy reveals a complex hierarchical pattern of growthwith inward differentiation along hair follicle lineages. Most BCC cells express the transcription factor KLF4 and are committed to terminal differentiation. A small CD200+ CD45- BCC subpopulation that represents 1.63 ± 1.11% of all BCC cells resides in small clusters at the tumor periphery. By using reproducible in vivo xenograft growth assays, we determined that tumor initiating cell frequencies approximate one per 1.5 million unsorted BCC cells. The CD200+ CD45- BCC subpopulation recreated BCC tumor growth in vivo with typical histological architecture and expression of sonic hedgehog-regulated genes. Reproducible in vivo BCC growth was achievedwith as few as 10,000 CD200+ CD45- cells, representing ̃1,500-fold enrichment. CD200- CD45- BCC cells were unable to form tumors. These findings establish a platform to study the effects of Smoothened antagonists on BCC tumor initiating cell and also suggest that currently available anti-CD200 therapy be considered, either asmonotherapy or an adjunct to Smoothened antagonists, in the treatment of inoperable BCC.

Original languageEnglish
Pages (from-to)1434-1439
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number4
Publication statusPublished - 2013 Jan 22
Externally publishedYes

ASJC Scopus subject areas

  • General


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