TY - GEN
T1 - CELL DEBRIS FILTERING AND LIQUID EXCHANGE USING NANOFLUDIC DEVICE FOR PRETREATMENT OF SINGLE CELL LYSATE SAMPLE
AU - Morikawa, Kyojiro
AU - Matsuura, Shu
AU - Kazoe, Yutaka
AU - Yoshizaki, Ayumi
AU - Kitamori, Takehiko
N1 - Funding Information:
We acknowledge financial support of CREST, JST.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - We developed methods for cell debris filtering and buffer exchange using nanofluidic devices. Shape of nanochannels were designed based on the principle of debris filtering and protein trapping. Using the devices, cell debris filtering and buffer exchange were successfully performed. These devices will be useful for single cell analysis in the point of pretreatment of lysate sample. In addition, the methods will provide new methodologies for transportation of solvent and solute in nanochannels, which will greatly contribute to micro-nanofluidics.
AB - We developed methods for cell debris filtering and buffer exchange using nanofluidic devices. Shape of nanochannels were designed based on the principle of debris filtering and protein trapping. Using the devices, cell debris filtering and buffer exchange were successfully performed. These devices will be useful for single cell analysis in the point of pretreatment of lysate sample. In addition, the methods will provide new methodologies for transportation of solvent and solute in nanochannels, which will greatly contribute to micro-nanofluidics.
KW - Filtering
KW - Nanochannel
KW - Nanofluidics
KW - Single Cell Analysis
UR - http://www.scopus.com/inward/record.url?scp=85136980777&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136980777&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136980777
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1409
EP - 1410
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -