Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells

Keita Kajikawa; Kent Imaizumi; Munehisa Shinozaki; Shinsuke Shibata; Tomoko Shindo; Takahiro Kitagawa; Reo Shibata; Yasuhiro Kamata; Kota Kojima; Narihito Nagoshi; Morio Matsumoto; Masaya Nakamura; Hideyuki Okano

doi:10.1186/s13041-020-00662-w

Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells

Keita Kajikawa, Kent Imaizumi, Munehisa Shinozaki, Shinsuke Shibata, Tomoko Shindo, Takahiro Kitagawa, Reo Shibata, Yasuhiro Kamata, Kota Kojima, Narihito Nagoshi, Morio Matsumoto, Masaya Nakamura, Hideyuki Okano

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.

Original language	English
Article number	120
Journal	Molecular brain
Volume	13
Issue number	1
DOIs	https://doi.org/10.1186/s13041-020-00662-w
Publication status	Published - 2020 Sept 3

Keywords

Region-specific neural progenitors
Spinal cord injury
Stem cell therapy

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

Access to Document

10.1186/s13041-020-00662-w

Cite this

@article{b05925c388174fa6818092ca931e1b20,

title = "Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells",

abstract = "The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.",

keywords = "Region-specific neural progenitors, Spinal cord injury, Stem cell therapy",

author = "Keita Kajikawa and Kent Imaizumi and Munehisa Shinozaki and Shinsuke Shibata and Tomoko Shindo and Takahiro Kitagawa and Reo Shibata and Yasuhiro Kamata and Kota Kojima and Narihito Nagoshi and Morio Matsumoto and Masaya Nakamura and Hideyuki Okano",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s).",

year = "2020",

month = sep,

day = "3",

doi = "10.1186/s13041-020-00662-w",

language = "English",

volume = "13",

journal = "Molecular brain",

issn = "1756-6606",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells

AU - Kajikawa, Keita

AU - Imaizumi, Kent

AU - Shinozaki, Munehisa

AU - Shibata, Shinsuke

AU - Shindo, Tomoko

AU - Kitagawa, Takahiro

AU - Shibata, Reo

AU - Kamata, Yasuhiro

AU - Kojima, Kota

AU - Nagoshi, Narihito

AU - Matsumoto, Morio

AU - Nakamura, Masaya

AU - Okano, Hideyuki

PY - 2020/9/3

Y1 - 2020/9/3

N2 - The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.

AB - The transplantation of neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) has beneficial effects on spinal cord injury (SCI). However, while there are many subtypes of NPCs with different regional identities, the subtype of iPSC-derived NPCs that is most appropriate for cell therapy for SCI has not been identified. Here, we generated forebrain- and spinal cord-type NPCs from human iPSCs and grafted them onto the injured spinal cord in mice. These two types of NPCs retained their regional identities after transplantation and exhibited different graft-host interconnection properties. NPCs with spinal cord regional identity but not those with forebrain identity resulted in functional improvement in SCI mice, especially in those with mild-to-moderate lesions. This study highlights the importance of the regional identity of human iPSC-derived NPCs used in cell therapy for SCI.

KW - Region-specific neural progenitors

KW - Spinal cord injury

KW - Stem cell therapy

UR - http://www.scopus.com/inward/record.url?scp=85090320357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85090320357&partnerID=8YFLogxK

U2 - 10.1186/s13041-020-00662-w

DO - 10.1186/s13041-020-00662-w

M3 - Article

C2 - 32883317

AN - SCOPUS:85090320357

SN - 1756-6606

VL - 13

JO - Molecular brain

JF - Molecular brain

IS - 1

M1 - 120

ER -

Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this