Cellular assemblages by using polymer chains possessing bio-affinities for tissue engineering

Makiko Kawada, Keiji Fujimoto

Research output: Contribution to conferencePaperpeer-review


Human umbilical vein endothelial cells were modified with polymer chains to display cell-adhesive sites through the ligand-receptor interaction on the cell surface. We prepared a poly (ethylene oxide) (PEO) having a cell-adhesive peptide Gly-Arg-Gly-Asp-Ser (GRGDS) at both ends to link among cells. The polymer chains were added to the HUVEC suspension to obtain cell agglomerates. SEM observations revealed that cells closely attached to form agglomerates. Cell agglomerates could adhere onto the collagen-coated PS plate and spread from their circumference. Eventually, cell shapes changed from agglomerates to a monolayer. We found that cell growth was facilitated when cells remained agglomerated and that the size and the morphologies of cell agglomerates significantly influenced on the cell proliferation.

Original languageEnglish
Number of pages1
Publication statusPublished - 2006 Oct 19
Event55th SPSJ Annual Meeting - Nagoya, Japan
Duration: 2006 May 242006 May 26


Other55th SPSJ Annual Meeting


  • Cell adhesion
  • Cell agglomerates
  • Poly(ethylene oxide) (PEO)
  • RGD
  • Tissue Engineering

ASJC Scopus subject areas

  • Engineering(all)


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