Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid

Zhi Hong Yang; Yoshiyuki Koyama; Toshinori Sato

Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid

Zhi Hong Yang, Yoshiyuki Koyama, Toshinori Sato

Department of Biosciences and Informatics

Research output: Contribution to conference › Paper › peer-review

Abstract

In this study, we investigated receptor-mediated transfection of the ternary nanoparticles in which a pDNA/chitosan particles is coated with hyaluronic acid (HA), that is known to specifically bind CD44. It was found that the coating of the pDNA/chiorsan complexes with HA depressed self-aggregation in BSA solution, and its cytotoxicity was very low. Although the ternary nanoparticles was negative charge, the gene expression of luciferase gene on CHO cells which express CD44 were improved, which is well agreed with the cell uptake efficiency.

Original language	English
Number of pages	1
Publication status	Published - 2006 Oct 19
Event	55th SPSJ Annual Meeting - Nagoya, Japan Duration: 2006 May 24 → 2006 May 26

Other

Other	55th SPSJ Annual Meeting
Country/Territory	Japan
City	Nagoya
Period	06/5/24 → 06/5/26

Keywords

Chitosan
Gene transfer
Hayluronic acid/CD44

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid",

abstract = "In this study, we investigated receptor-mediated transfection of the ternary nanoparticles in which a pDNA/chitosan particles is coated with hyaluronic acid (HA), that is known to specifically bind CD44. It was found that the coating of the pDNA/chiorsan complexes with HA depressed self-aggregation in BSA solution, and its cytotoxicity was very low. Although the ternary nanoparticles was negative charge, the gene expression of luciferase gene on CHO cells which express CD44 were improved, which is well agreed with the cell uptake efficiency.",

keywords = "Chitosan, Gene transfer, Hayluronic acid/CD44",

author = "Yang, {Zhi Hong} and Yoshiyuki Koyama and Toshinori Sato",

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T1 - Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid

AU - Yang, Zhi Hong

AU - Koyama, Yoshiyuki

AU - Sato, Toshinori

PY - 2006/10/19

Y1 - 2006/10/19

N2 - In this study, we investigated receptor-mediated transfection of the ternary nanoparticles in which a pDNA/chitosan particles is coated with hyaluronic acid (HA), that is known to specifically bind CD44. It was found that the coating of the pDNA/chiorsan complexes with HA depressed self-aggregation in BSA solution, and its cytotoxicity was very low. Although the ternary nanoparticles was negative charge, the gene expression of luciferase gene on CHO cells which express CD44 were improved, which is well agreed with the cell uptake efficiency.

AB - In this study, we investigated receptor-mediated transfection of the ternary nanoparticles in which a pDNA/chitosan particles is coated with hyaluronic acid (HA), that is known to specifically bind CD44. It was found that the coating of the pDNA/chiorsan complexes with HA depressed self-aggregation in BSA solution, and its cytotoxicity was very low. Although the ternary nanoparticles was negative charge, the gene expression of luciferase gene on CHO cells which express CD44 were improved, which is well agreed with the cell uptake efficiency.

KW - Chitosan

KW - Gene transfer

KW - Hayluronic acid/CD44

UR - http://www.scopus.com/inward/record.url?scp=33749827451&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749827451&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33749827451

T2 - 55th SPSJ Annual Meeting

Y2 - 24 May 2006 through 26 May 2006

ER -

Characterization and cell transfection of pDNA/chitosan nanoparticles coated with hyaluronic acid

Abstract

Other

Keywords

ASJC Scopus subject areas

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