Characterization of bovine lactoferrin nanoparticle prepared by desolvation technique

Kazuaki Taguchi, Victor Tuan Giam Chuang, Mai Hashimoto, Mayuki Nakayama, Mina Sakuragi, Yuki Enoki, Koji Nishi, Kazuaki Matsumoto, Hakaru Seo, Masaki Otagiri, Keishi Yamasaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Lactoferrin (Lf) nanoparticles have been developed as a carrier of drugs and gene. Two main methods, desolvation technique and emulsification method, for preparation of protein nanoparticles have been reported so far, but most of the previous reports of Lf nanoparticles preparation are limited to emulsification method. In this study, we investigated the optimal conditions by desolvation technique for the preparation of glutaraldehyde-crosslinked bovine Lf (bLf) nanoparticles within the size range of 100–200nm, and evaluated their properties as a carrier for oral and intravenous drug delivery. The experimental results of dynamic light scattering and Transmission Electron Microscope suggested that glutaraldehyde-crosslinked bLf nanoparticles with 150nm in size could be produced by addition of 2-propanol as the desolvating solvent into the bLf solution adjusted to pH 6, followed by crosslinking with glutaraldehyde. These cross-linked bLf nanoparticles were found to be compatible to blood components and resistant against rapid degradation by pepsin. Thus, cross-linked bLf nanoparticles prepared by desolvation technique can be applied as a drug carrier for intravenous administration and oral delivery.

Original languageEnglish
Pages (from-to)766-772
Number of pages7
JournalChemical and Pharmaceutical Bulletin
Issue number8
Publication statusPublished - 2020


  • Desolvation technique
  • Lactoferrin
  • Nanoparticle

ASJC Scopus subject areas

  • General Chemistry
  • Drug Discovery


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