TY - JOUR
T1 - Characterization of cDNA encoding full-length mouse platelet glycoprotein IX
AU - Kitaguchi, T.
AU - Murata, M.
AU - Ambo, H.
AU - Ikeda, Y.
PY - 1998
Y1 - 1998
N2 - Glycoprotein (GP) IX is a platelet membrane 20 kD protein, which is associated with GPIba, GPIbβ, and GPV to form GPIb/IX/V complex. GPIb/IX/V complex is a major receptor for yon Willebrand factor, which mediates platelet adhesion and aggregation under high shear stress conditions. The relevance of this receptor for hemostasis has been implicated by a congenital bleeding disorder lacking the receptor, Bernard-Soulier syndrome. All subunits for the human receptor have been cloned and characterized. However, the function of GPIX is still elusive. To obtain further information of GPIX, we have determined a cDNA sequence of mouse GPIX (811 bp). The deduced amino- acid sequence (177aa) was 71% identical to the human GPIX protein. All cysteine residues in extracytoplasmic domain and putative N-linked glycosylation site (Asn44) were conserved. Mouse GPIX contained a leucine- rich glycoprotein sequence composed of 24 amino acids, as did human GPIX.
AB - Glycoprotein (GP) IX is a platelet membrane 20 kD protein, which is associated with GPIba, GPIbβ, and GPV to form GPIb/IX/V complex. GPIb/IX/V complex is a major receptor for yon Willebrand factor, which mediates platelet adhesion and aggregation under high shear stress conditions. The relevance of this receptor for hemostasis has been implicated by a congenital bleeding disorder lacking the receptor, Bernard-Soulier syndrome. All subunits for the human receptor have been cloned and characterized. However, the function of GPIX is still elusive. To obtain further information of GPIX, we have determined a cDNA sequence of mouse GPIX (811 bp). The deduced amino- acid sequence (177aa) was 71% identical to the human GPIX protein. All cysteine residues in extracytoplasmic domain and putative N-linked glycosylation site (Asn44) were conserved. Mouse GPIX contained a leucine- rich glycoprotein sequence composed of 24 amino acids, as did human GPIX.
KW - CDNA
KW - Glycoprotein IX
KW - Leucine-rich glycoprotein
KW - Mouse
KW - Platelets
KW - Receptor
KW - Von Willebrand factor
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U2 - 10.1097/00001721-199806000-00011
DO - 10.1097/00001721-199806000-00011
M3 - Article
C2 - 9690810
AN - SCOPUS:0031803302
SN - 0957-5235
VL - 9
SP - 381
EP - 385
JO - Blood Coagulation and Fibrinolysis
JF - Blood Coagulation and Fibrinolysis
IS - 4
ER -