TY - JOUR
T1 - Characterization of fifteen microsatellite markers for the Japanese fire-bellied newt, Cynops pyrrhogaster
AU - Tominaga, Atsushi
AU - Matsui, Masafumi
AU - Yoshikawa, Natsuhiko
AU - Hayano, Azusa
AU - Inoue-Murayama, Miho
N1 - Funding Information:
Acknowledgments This work was conducted under the Cooperation Research Program of Wildlife Research Center, Kyoto University, and was partly supported by a research fund from Kyoto University and Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan, through the Japan Society for the Promotion of Science to MM (Nos. 20510215, 23510294) and AT (No. 22770087, 25870572).
PY - 2014/6
Y1 - 2014/6
N2 - The Japanese fire-bellied newt Cynops pyrrhogaster, endemic to mainland Japan is ranked as Near Threatened in the red list of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed fifteen novel microsatellite markers for this species using expressed sequence tag and next-generation sequencing data. The number of alleles and expected heterozygosity at each locus were 3-28 (mean = 11.6) and 0-0.97. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of C. pyrrhogaster.
AB - The Japanese fire-bellied newt Cynops pyrrhogaster, endemic to mainland Japan is ranked as Near Threatened in the red list of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed fifteen novel microsatellite markers for this species using expressed sequence tag and next-generation sequencing data. The number of alleles and expected heterozygosity at each locus were 3-28 (mean = 11.6) and 0-0.97. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of C. pyrrhogaster.
KW - Conservation
KW - Cynops pyrrhogaster
KW - Expressed sequence tag
KW - Genetic diversity
KW - Microsatellite
KW - Next-generation sequencing
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U2 - 10.1007/s12686-013-0104-5
DO - 10.1007/s12686-013-0104-5
M3 - Letter
AN - SCOPUS:84900807225
SN - 1877-7252
VL - 6
SP - 405
EP - 407
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -