TY - JOUR
T1 - Characterization of nineteen microsatellite markers for the Japanese clouded salamander, Hynobius nebulosus, using the NGS
AU - Yoshikawa, Natsuhiko
AU - Matsui, Masafumi
AU - Inoue-Murayama, Miho
N1 - Funding Information:
Acknowledgments The educatory committees of relevant local governments granted permission to study protected Japanese clouded salamander. We thank K. Nishikawa, S. Tanabe, and N. Wada for help in collecting specimens, and A. Hayano for assisting with the experiment. This work was conducted under the Cooperation Research Program of Wildlife Research Center, Kyoto University, and was partly supported by a research fund from Kyoto University and Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan, through the Japan Society for the Promotion of Science to MM (Nos. 20510215, 23510294).
PY - 2013/9
Y1 - 2013/9
N2 - The Japanese clouded salamander Hynobius nebulosus, endemic to Western Japan, is ranked as vulnerable in the Red List of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed 19 novel microsatellite markers for H. nebulosus. The number of alleles and expected heterozygosity at each locus were 3-14 (mean = 6.63) and 0.22-0.82, respectively, in this species. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of H. nebulosus.
AB - The Japanese clouded salamander Hynobius nebulosus, endemic to Western Japan, is ranked as vulnerable in the Red List of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed 19 novel microsatellite markers for H. nebulosus. The number of alleles and expected heterozygosity at each locus were 3-14 (mean = 6.63) and 0.22-0.82, respectively, in this species. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of H. nebulosus.
KW - Conservation
KW - Genetic diversity
KW - Hynobius nebulosus
KW - Microsatellite
KW - Next-generation sequencing
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U2 - 10.1007/s12686-013-9861-4
DO - 10.1007/s12686-013-9861-4
M3 - Article
AN - SCOPUS:84880971440
SN - 1877-7252
VL - 5
SP - 603
EP - 605
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -