Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

Yuki Haraguchi; Kanako Kuwasako; Yutaka Muto; Yoshitaka Bessho; Madoka Nishimoto; Shigeyuki Yokoyama; Akio Kanai; Gota Kawai; Taiichi Sakamoto

doi:10.1093/nass/nrp133

Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

Yuki Haraguchi
, Kanako Kuwasako
, Yutaka Muto
, Yoshitaka Bessho
, Madoka Nishimoto
, Shigeyuki Yokoyama
, Akio Kanai
, Gota Kawai
, Taiichi Sakamoto

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.

Original language	English
Pages (from-to)	265-266
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	53
DOIs	https://doi.org/10.1093/nass/nrp133
Publication status	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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abstract = "SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.",

author = "Yuki Haraguchi and Kanako Kuwasako and Yutaka Muto and Yoshitaka Bessho and Madoka Nishimoto and Shigeyuki Yokoyama and Akio Kanai and Gota Kawai and Taiichi Sakamoto",

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T1 - Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

AU - Haraguchi, Yuki

AU - Kuwasako, Kanako

AU - Muto, Yutaka

AU - Bessho, Yoshitaka

AU - Nishimoto, Madoka

AU - Yokoyama, Shigeyuki

AU - Kanai, Akio

AU - Kawai, Gota

AU - Sakamoto, Taiichi

PY - 2009

Y1 - 2009

N2 - SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.

AB - SELEX is a conventional method to obtain high affinity nucleic acids to target molecules. In this study, high affinity RNA molecules against SRP19 protein were selected by using a randomized library. The primary and predicted secondary structures of the aptamers are different from those of S-domain RNA which is the natural target of SRP19 protein. Comparison of structural features between S-domain RNA and aptamers might enhance our understanding on RNA-protein interaction.

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UR - https://www.scopus.com/pages/publications/77952506567#tab=citedBy

U2 - 10.1093/nass/nrp133

DO - 10.1093/nass/nrp133

M3 - Article

C2 - 19749362

AN - SCOPUS:77952506567

SN - 0261-3166

SP - 265

EP - 266

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 53

ER -

Characterization of RNA aptamers against SRP19 protein having sequences different from SRP RNA.

Abstract

ASJC Scopus subject areas

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