Characterization of the cell of origin and propagation potential of the fibroblast growth factor 9-induced mouse model of lung adenocarcinoma

Daisuke Arai, Ahmed E. Hegab, Kenzo Soejima, Aoi Kuroda, Kota Ishioka, Hiroyuki Yasuda, Katsuhiko Naoki, Shizuko Kagawa, Junko Hamamoto, Yongjun Yin, David M. Ornitz, Tomoko Betsuyaku

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Fibroblast growth factor 9 (FGF9) is essential for lung development and is highly expressed in a subset of human lung adenocarcinomas. We recently described a mouse model in which FGF9 expression in the lung epithelium caused proliferation of the airway epithelium at the terminal bronchioles and led to rapid development of adenocarcinoma. Here, we used this model to characterize the effects of prolonged FGF9 induction on the proximal and distal lung epithelia, and examined the propagation potential of FGF9-induced lung tumours. We showed that prolonged FGF9 over-expression in the lung resulted in the development of adenocarcinomas arising from both alveolar type II and airway secretory cells in the lung parenchyma and airways, respectively. We found that tumour cells harboured tumour-propagating cells that were able to form secondary tumours in recipient mice, regardless of FGF9 expression. However, the highest degree of tumour propagation was observed when unfractionated tumour cells were co-administered with autologous, tumour-associated mesenchymal cells. Although the initiation of lung adenocarcinomas was dependent on activation of the FGF9-FGF receptor 3 (FGFR3) signalling axis, maintenance and propagation of the tumour was independent of this signalling. Activation of an alternative FGF-FGFR axis and the interaction with tumour stromal cells is likely to be responsible for the development of this independence. This study demonstrates the complex role of FGF-FGFR signalling in the initiation, growth and propagation of lung cancer. Our findings suggest that analysing the expressions of FGF-FGFRs in human lung cancer will be a useful tool for guiding customized therapy.

Original languageEnglish
Pages (from-to)593-605
Number of pages13
JournalJournal of Pathology
Issue number4
Publication statusPublished - 2015 Mar 1


  • Adenocarcinoma
  • FGF9
  • FGFR
  • Lung cancer
  • Tumour propagation
  • Tumour-associated lung fibroblasts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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