TY - JOUR
T1 - Characterization of two acidic polysaccharides having immunological activities from the root of panax ginseng
AU - Tomoda, Masashi
AU - Takeda, Kenji
AU - Shimizu, Noriko
AU - Gonda, Ryōko
AU - Ōhara, Naoko
AU - Takada, Katsutoshi
AU - Hirabayashi, Keiko
PY - 1993
Y1 - 1993
N2 - Two acidic polysaccharides, named ginsenan PA and ginsenan PB, were isolated from the root of Panax ginseng C. A. MEYER. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 1.6×105 and 5.5×104, respectively. They are composed of L-arabinose : D-galactose : L-rhamnose : D-galacturonic acid : D-glucuronic acid in the molar ratios of 11 : 22 : 1 : 6 : 1 (ginsenan PA) and 3 : 7 : 2 : 8 : 1 (ginsenan PB), in addition to small amounts of O-acetyl groups. Almost all (ginsenan PA) and part (ginsenan PB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly both α-arabino-β-3, 6-galactan type and rhamnogalacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test, pronounced anti-complementary activity and alkaline phosphatase-inducing activity in a dose dependent manner.
AB - Two acidic polysaccharides, named ginsenan PA and ginsenan PB, were isolated from the root of Panax ginseng C. A. MEYER. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 1.6×105 and 5.5×104, respectively. They are composed of L-arabinose : D-galactose : L-rhamnose : D-galacturonic acid : D-glucuronic acid in the molar ratios of 11 : 22 : 1 : 6 : 1 (ginsenan PA) and 3 : 7 : 2 : 8 : 1 (ginsenan PB), in addition to small amounts of O-acetyl groups. Almost all (ginsenan PA) and part (ginsenan PB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly both α-arabino-β-3, 6-galactan type and rhamnogalacturonan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test, pronounced anti-complementary activity and alkaline phosphatase-inducing activity in a dose dependent manner.
KW - Panax ginseng
KW - acidic arabinogalactan
KW - ginsenan PA
KW - ginsenan PB
KW - immunological activity
KW - polysaccharide structure
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U2 - 10.1248/bpb.16.22
DO - 10.1248/bpb.16.22
M3 - Article
C2 - 8369746
AN - SCOPUS:0027481801
SN - 0918-6158
VL - 16
SP - 22
EP - 25
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 1
ER -