TY - JOUR
T1 - Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos
AU - Oelgeschläger, Michael
AU - Kuroda, Hiroki
AU - Reversade, Bruno
AU - De Robertis, E. M.
N1 - Funding Information:
We thank Drs. I. Dawid, F. Watt, and C.H. Heldin for materials, C. Coffinier, E. Pera, O. Wessely, and J. Larraı́n for comments on the manuscript, and U. Tran and A. Cuellar for technical assistance. M.O. was an HFSPO postdoctoral fellow. E.M.D.R. is an Investigator of the Howard Hughes Medical Institute. This work was supported by the National Institutes of Health (R37 HD21502-16).
PY - 2003/2/1
Y1 - 2003/2/1
N2 - We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.
AB - We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.
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U2 - 10.1016/S1534-5807(02)00404-5
DO - 10.1016/S1534-5807(02)00404-5
M3 - Article
C2 - 12586065
AN - SCOPUS:0037321789
SN - 1534-5807
VL - 4
SP - 219
EP - 230
JO - Developmental Cell
JF - Developmental Cell
IS - 2
ER -