Chromatographic separation and identification of human urine components

Y. Nagata; Y. Matsushima; H. Ugai; M. Kikuchi; N. Takai

doi:10.1007/BF02688497

Chromatographic separation and identification of human urine components

Y. Nagata, Y. Matsushima, H. Ugai, M. Kikuchi, N. Takai

School of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Ultraviolet-absorbing components of human urine were separated by HPLC with a large scale column (250 × 28 mm I.D. stainless-steel) containing a macroreticular type strongly basic anion-exchange resin. The sample was eluted with a linear gradient of 0.006 M to 6.0 M ammonium acetate buffer (pH 4.40) and different fractions were collected. The identification of one compound using ultraviolet, infrared and mass spectral data is illustrated; it was found to be uric acid.

Original language	English
Pages (from-to)	317-318
Number of pages	2
Journal	Chromatographia
Volume	24
Issue number	1
DOIs	https://doi.org/10.1007/BF02688497
Publication status	Published - 1987 Dec

Keywords

Human urine
Large scale column liquid chromatography
Strongly basic anion-exchange resin
Uric acid

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Organic Chemistry

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10.1007/BF02688497

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author = "Y. Nagata and Y. Matsushima and H. Ugai and M. Kikuchi and N. Takai",

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AU - Ugai, H.

AU - Kikuchi, M.

AU - Takai, N.

PY - 1987/12

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KW - Large scale column liquid chromatography

KW - Strongly basic anion-exchange resin

KW - Uric acid

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Chromatographic separation and identification of human urine components

Abstract

Keywords

ASJC Scopus subject areas

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