Cleavable linker for photo-cross-linked small-molecule affinity matrix

Naoki Kanoh; Hiroshi Takayama; Kaori Honda; Takashi Moriya; Takayuki Teruya; Siro Simizu; Hiroyuki Osada; Yoshiharu Iwabuchi

doi:10.1021/bc900316q

Cleavable linker for photo-cross-linked small-molecule affinity matrix

Naoki Kanoh, Hiroshi Takayama, Kaori Honda, Takashi Moriya, Takayuki Teruya, Siro Simizu, Hiroyuki Osada, Yoshiharu Iwabuchi

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

Original language	English
Pages (from-to)	182-186
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	21
Issue number	1
DOIs	https://doi.org/10.1021/bc900316q
Publication status	Published - 2010 Jan 20
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

Access to Document

10.1021/bc900316q

Cite this

@article{6fed2587fb99476caa4d028cb429c20e,

title = "Cleavable linker for photo-cross-linked small-molecule affinity matrix",

abstract = "The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.",

author = "Naoki Kanoh and Hiroshi Takayama and Kaori Honda and Takashi Moriya and Takayuki Teruya and Siro Simizu and Hiroyuki Osada and Yoshiharu Iwabuchi",

year = "2010",

month = jan,

day = "20",

doi = "10.1021/bc900316q",

language = "English",

volume = "21",

pages = "182--186",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - Cleavable linker for photo-cross-linked small-molecule affinity matrix

AU - Kanoh, Naoki

AU - Takayama, Hiroshi

AU - Honda, Kaori

AU - Moriya, Takashi

AU - Teruya, Takayuki

AU - Simizu, Siro

AU - Osada, Hiroyuki

AU - Iwabuchi, Yoshiharu

PY - 2010/1/20

Y1 - 2010/1/20

N2 - The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

AB - The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

UR - http://www.scopus.com/inward/record.url?scp=74949128893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74949128893&partnerID=8YFLogxK

U2 - 10.1021/bc900316q

DO - 10.1021/bc900316q

M3 - Article

C2 - 20028022

AN - SCOPUS:74949128893

SN - 1043-1802

VL - 21

SP - 182

EP - 186

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 1

ER -

Cleavable linker for photo-cross-linked small-molecule affinity matrix

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this