Cloning and characterization of mouse mSox13 cDNA

Susumu Kido; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Yasunori Yoshimura; Sadakazu Aiso

doi:10.1016/S0378-1119(97)00667-7

Cloning and characterization of mouse mSox13 cDNA

Susumu Kido, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Yasunori Yoshimura, Sadakazu Aiso

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	Gene
Volume	208
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(97)00667-7
Publication status	Published - 1998 Feb 22
Externally published	Yes

Keywords

DNA binding
Embryo
HMG box
Leucine zipper
SRY

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(97)00667-7

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title = "Cloning and characterization of mouse mSox13 cDNA",

abstract = "A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.",

keywords = "DNA binding, Embryo, HMG box, Leucine zipper, SRY",

author = "Susumu Kido and Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Yasunori Yoshimura and Sadakazu Aiso",

note = "Funding Information: We thank Dr H. Ikeshima for her generous gift of the mouse cDNA library. This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A., Grants-in-Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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doi = "10.1016/S0378-1119(97)00667-7",

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T1 - Cloning and characterization of mouse mSox13 cDNA

AU - Kido, Susumu

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Yoshimura, Yasunori

AU - Aiso, Sadakazu

N1 - Funding Information: We thank Dr H. Ikeshima for her generous gift of the mouse cDNA library. This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A., Grants-in-Aids from Ministry of Education, Science and Culture to S.A. and Y.H., and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1998/2/22

Y1 - 1998/2/22

N2 - A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.

AB - A novel SRY-related cDNA, mSox13, was isolated from a λ phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.

KW - DNA binding

KW - Embryo

KW - HMG box

KW - Leucine zipper

KW - SRY

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Cloning and characterization of mouse mSox13 cDNA

Abstract

Keywords

ASJC Scopus subject areas

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