Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

Masanobu Kawasaki; Shinichi Uchida; Toshiaki Monkawa; Atsushi Miyawaki; Katsuhiko Mikoshiba; Fumiaki Marumo; Sei Sasaki

doi:10.1016/0896-6273(94)90215-1

Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

Masanobu Kawasaki, Shinichi Uchida, Toshiaki Monkawa, Atsushi Miyawaki, Katsuhiko Mikoshiba, Fumiaki Marumo, Sei Sasaki

Medical Education Center

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219 Citations (Scopus)

Abstract

cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

Original language	English
Pages (from-to)	597-604
Number of pages	8
Journal	Neuron
Volume	12
Issue number	3
DOIs	https://doi.org/10.1016/0896-6273(94)90215-1
Publication status	Published - 1994 Mar

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0896-6273(94)90215-1

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title = "Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells",

abstract = "cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.",

author = "Masanobu Kawasaki and Shinichi Uchida and Toshiaki Monkawa and Atsushi Miyawaki and Katsuhiko Mikoshiba and Fumiaki Marumo and Sei Sasaki",

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T1 - Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

AU - Kawasaki, Masanobu

AU - Uchida, Shinichi

AU - Monkawa, Toshiaki

AU - Miyawaki, Atsushi

AU - Mikoshiba, Katsuhiko

AU - Marumo, Fumiaki

AU - Sasaki, Sei

PY - 1994/3

Y1 - 1994/3

N2 - cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

AB - cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

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Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

Abstract

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