Coculture of a Pathogenic Actinomycete and Animal Cells to Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect

Yasumasa Hara; Midori A. Arai; Kazufumi Toume; Hyuma Masu; Tomoyuki Sato; Katsuko Komatsu; Takashi Yaguchi; Masami Ishibashi

doi:10.1021/acs.orglett.8b02522

Coculture of a Pathogenic Actinomycete and Animal Cells to Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect

Yasumasa Hara
, Midori A. Arai
, Kazufumi Toume
, Hyuma Masu
, Tomoyuki Sato
, Katsuko Komatsu
, Takashi Yaguchi
, Masami Ishibashi

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554^T and the mouse macrophage-like cell line J774.1 in a modified Czapek-Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.

Original language	English
Pages (from-to)	5831-5834
Number of pages	4
Journal	Organic Letters
Volume	20
Issue number	18
DOIs	https://doi.org/10.1021/acs.orglett.8b02522
Publication status	Published - 2018 Sept 21
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/acs.orglett.8b02522

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title = "Coculture of a Pathogenic Actinomycete and Animal Cells to Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect",

abstract = "A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554T and the mouse macrophage-like cell line J774.1 in a modified Czapek-Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.",

author = "Yasumasa Hara and Arai, \{Midori A.\} and Kazufumi Toume and Hyuma Masu and Tomoyuki Sato and Katsuko Komatsu and Takashi Yaguchi and Masami Ishibashi",

note = "Funding Information: This study was supported by JSPS KAKENHI Grant No. 17H03992 and the Strategic Priority Research Promotion Program of Chiba University, “Phytochemical Plant Molecular Sciences”. Publisher Copyright: Copyright {\textcopyright} 2018 American Chemical Society.",

year = "2018",

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doi = "10.1021/acs.orglett.8b02522",

language = "English",

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T1 - Coculture of a Pathogenic Actinomycete and Animal Cells to Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect

AU - Hara, Yasumasa

AU - Arai, Midori A.

AU - Toume, Kazufumi

AU - Masu, Hyuma

AU - Sato, Tomoyuki

AU - Komatsu, Katsuko

AU - Yaguchi, Takashi

AU - Ishibashi, Masami

N1 - Funding Information: This study was supported by JSPS KAKENHI Grant No. 17H03992 and the Strategic Priority Research Promotion Program of Chiba University, “Phytochemical Plant Molecular Sciences”. Publisher Copyright: Copyright © 2018 American Chemical Society.

PY - 2018/9/21

Y1 - 2018/9/21

N2 - A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554T and the mouse macrophage-like cell line J774.1 in a modified Czapek-Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.

AB - A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554T and the mouse macrophage-like cell line J774.1 in a modified Czapek-Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.

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U2 - 10.1021/acs.orglett.8b02522

DO - 10.1021/acs.orglett.8b02522

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AN - SCOPUS:85052932092

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ER -

Coculture of a Pathogenic Actinomycete and Animal Cells to Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect

Abstract

ASJC Scopus subject areas

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