TY - JOUR
T1 - Coffee induces vascular endothelial growth factor (VEGF) expression in human neuroblastama SH-SY5Y cells
AU - Kakio, Shota
AU - Funakoshi-Tago, Megumi
AU - Kobata, Kenji
AU - Tamura, Hiroomi
N1 - Funding Information:
We are grateful to Dr K. Oka for his encouragement. This work was supported partially by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan, and by a grant from the MEXT-Supported Program for the Strategic Research Foundation at Private Universities.
Publisher Copyright:
© 2016 Taylor & Francis.
PY - 2017/7/3
Y1 - 2017/7/3
N2 - Recent evidence indicates that hypoxia-inducible vascular endothelial growth factor (VEGF) has neurotrophic and neuroprotective effects on neuronal and glial cells. On the other hand, recent epidemiological studies showed that daily coffee consumption has been associated with a lower risk of several neuronal disorders. Therefore, we investigated the effect of coffee on VEGF expression in human neuroblastoma SH-SY5Y cells. We found that even low concentration of coffee (<2%) strongly induced VEGF expression via an activation of HIF-1α. The activation of HIF-1α by coffee was attributed to the coffee-dependent inhibition of prolyl hydroxylation of HIF1α, which is essential for proteolytic degradation of HIF-1α. However, no inhibition was observed at the catalytic activity in vitro. Coffee component(s) responsible for the activation of HIF-1α was not major constituents such as caffeine, caffeic acid, chlorogenic acid, and trigonelline, but was found to emerge during roasting process. The active component(s) was extractable with ethyl acetate. Our results suggest that daily consumption of coffee may induce VEGF expression in neuronal cells. This might be related to protective effect of coffee on neural disorders such as Alzheimer's disease and Parkinson's disease.
AB - Recent evidence indicates that hypoxia-inducible vascular endothelial growth factor (VEGF) has neurotrophic and neuroprotective effects on neuronal and glial cells. On the other hand, recent epidemiological studies showed that daily coffee consumption has been associated with a lower risk of several neuronal disorders. Therefore, we investigated the effect of coffee on VEGF expression in human neuroblastoma SH-SY5Y cells. We found that even low concentration of coffee (<2%) strongly induced VEGF expression via an activation of HIF-1α. The activation of HIF-1α by coffee was attributed to the coffee-dependent inhibition of prolyl hydroxylation of HIF1α, which is essential for proteolytic degradation of HIF-1α. However, no inhibition was observed at the catalytic activity in vitro. Coffee component(s) responsible for the activation of HIF-1α was not major constituents such as caffeine, caffeic acid, chlorogenic acid, and trigonelline, but was found to emerge during roasting process. The active component(s) was extractable with ethyl acetate. Our results suggest that daily consumption of coffee may induce VEGF expression in neuronal cells. This might be related to protective effect of coffee on neural disorders such as Alzheimer's disease and Parkinson's disease.
KW - Coffee
KW - HIF-1a
KW - Neurodegeneration
KW - SH-SY5Y
KW - VEGF
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U2 - 10.1080/1028415X.2015.1133106
DO - 10.1080/1028415X.2015.1133106
M3 - Article
C2 - 26788968
AN - SCOPUS:84978525779
SN - 1028-415X
VL - 20
SP - 336
EP - 342
JO - Nutritional Neuroscience
JF - Nutritional Neuroscience
IS - 6
ER -
