Color correction in whole slide digital pathology

Yuri Murakami, Hikaru Gunji, Fumikazu Kimura, Masahiro Yamaguchi, Yoshiko Yamashita, Akira Saito, Tokiya Abe, Michiie Sakamoto, Pinky A. Bautista, Yukako Yagi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)


Whole slide imaging (WSI) is a technique with both the scanning and viewing of digital images of the entire area of a glass pathology slide. The implementation of WSI has started to accelerate, and the usages of WSI are expected for variety of applications such as image analysis, education, conference, and remote diagnosis. However, for implementation of WSI to clinical applications, color variation in digital pathology images is one of the most important issues. The reasons for color variation of whole slide images are mainly in the two processes: staining tissue samples and scanning glass slides. This paper presents the methods and the results of the color correction of whole slide images, with consideration of these two reasons. For color variation caused by scanning devices, the characterization of the whole slide scanner was performed based on a traditional empirical model using a known color chart, where the color chart is a miniature transparent one arranged on a glass slide. In addition, it was also tested that color correction using Hematoxylin and Eosin stained mouse embryo slide instead of the color chart slide. For the color variation caused by staining conditions, the color distribution of images was corrected so as to fit to that of a reference image. Such correction is relatively easy for pathology image, because the color distribution of a stained pathology slide presents a characteristic color distribution decided by its staining method. The effectiveness of the color correction methods were confirmed for whole slide images of tissue samples stained by some typical staining methods.

Original languageEnglish
Title of host publication20th Color and Imaging Conference
Subtitle of host publicationColor Science and Engineering Systems, Technologies, and Applications - Final Program and Proceedings, CIC 2012
Number of pages6
Publication statusPublished - 2012 Dec 1
Event20th Color and Imaging Conference: Color Science and Engineering Systems, Technologies, and Applications, CIC 2012 - Los Angeles, CA, United States
Duration: 2012 Nov 122012 Nov 16

Publication series

NameFinal Program and Proceedings - IS and T/SID Color Imaging Conference
ISSN (Print)1083-1304


Other20th Color and Imaging Conference: Color Science and Engineering Systems, Technologies, and Applications, CIC 2012
Country/TerritoryUnited States
CityLos Angeles, CA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


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