Combined Gene Expression and Chromatin Immunoprecipitation From a Single Mouse Hippocampus

Jean Christophe Helbling, Kenichiro Kinouchi, Pierre Trifilieff, Paolo Sassone-Corsi, Marie Pierre Moisan

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

All neuronal cells hold the same genetic information but vary by their structural and functional plasticity depending on the brain area and environmental influences. Such variability involves specific gene regulation, which is driven by transcription factors (TFs). In the field of neuroscience, epigenetics is the main mechanism that has been investigated to understand the dynamic modulation of gene expression by behavioral responses, stress responses, memory processes, etc. Nowadays, gene expression analyzed by real-time quantitative PCR and TF binding estimated by chromatin immunoprecipitation (ChIP) enables one to dissect this regulation. Because of the wide range of transgenic models, as well as cost-effective aspects, mouse models are widely used neuroscience. Thus, we have set up a protocol that allows extraction of both RNA for gene expression analysis and chromatin for ChIP experiment from a single mouse hippocampus. Using such protocols, information regarding gene expression and regulatory molecular mechanisms from the same animal can be integrated and correlated with neurobiological and behavioral outcomes.

Original languageEnglish
Article numbere33
JournalCurrent Protocols
Volume1
Issue number2
DOIs
Publication statusPublished - 2021 Feb

Keywords

  • ChIP
  • epigenetic
  • gene expression
  • hippocampus
  • mouse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Health Informatics
  • Medical Laboratory Technology
  • Medicine(all)

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