Common T cell receptor clonotype in lacrimal glands and labial salivary glands from patients with Sjögren's syndrome

Isao Matsumoto, Kazuo Tsubota, Yoshiyuki Satake, Yasuhiko Kita, Ryutaro Matsumura, Hideyuki Murata, Takashi Namekawa, Kusuki Nishioka, Itsuo Iwamoto, Yasushi Saitoh, Takayuki Sumida

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)


Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration into lacrimal and salivary glands leading to symptomatic dry eyes and mouth. Immunohistological studies have clarified that the majority of infiltrating lymphocytes around the lacrimal glands and labial salivary glands are CD4 positive αβ T cells. To analyze the pathogenesis of T cells infiltrating into lacrimal and labial salivary glands, we examined T cell clonotype of these cells in both glands from four SS patients using PCR-single-strand conformation polymorphism (SSCP) and a sequencing method. SSCP analysis showed that some infiltrating T cells in both glands expand clonally, suggesting that the cells proliferate by antigen-driven stimulation. Intriguingly, six to sixteen identical T cell receptor (TCR) Vβ genes were commonly found in lacrimal glands and labial salivary glands from individual patients. This indicates that some T cells infiltrating into both glands recognize the shared epitopes on autoantigens. Moreover, highly conserved amino acid sequence motifs were found in the TCR CDR3 region bearing the same TCR Vβ family gene from four SS patients, supporting the notion that the shared epitopes on antigens are limited. In conclusion, these findings suggest that some autoreactive T cells infiltrating into the lips and eyes recognize restricted epitopes of a common autoantigen in patients with SS.

Original languageEnglish
Pages (from-to)1969-1977
Number of pages9
JournalJournal of Clinical Investigation
Issue number8
Publication statusPublished - 1996 Apr 15
Externally publishedYes


  • Labial salivary glands
  • Lacrimal glands
  • SSCP
  • Sjögren's syndrome
  • T cell receptor Vβ gene

ASJC Scopus subject areas

  • General Medicine


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