TY - JOUR
T1 - Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes
AU - Qiu, Dongru
AU - Fujita, Kyoko
AU - Sakuma, Yuko
AU - Tanaka, Teruo
AU - Ohashi, Yoshiaki
AU - Ohshima, Hideyuki
AU - Tomita, Masaru
AU - Itaya, Mitsuhiro
PY - 2004/10
Y1 - 2004/10
N2 - The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons (rrn) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPβ, skin element, and PBSX, as well as the insertion element IS4Bsu1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.
AB - The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons (rrn) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPβ, skin element, and PBSX, as well as the insertion element IS4Bsu1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.
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U2 - 10.1128/AEM.70.10.6247-6256.2004
DO - 10.1128/AEM.70.10.6247-6256.2004
M3 - Article
C2 - 15466572
AN - SCOPUS:8144229668
SN - 0099-2240
VL - 70
SP - 6247
EP - 6256
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 10
ER -