TY - JOUR
T1 - Comparison between electrochemiluminescence of luminol and electrocatalysis by Prussian blue for the detection of hydrogen peroxide
AU - Rahmawati, Isnaini
AU - Fiorani, Andrea
AU - Irkham, None
AU - Wahyuni, Wulan Tri
AU - Wahyuono, Ruri Agung
AU - Einaga, Yasuaki
AU - Ivandini, Tribidasari A.
N1 - Publisher Copyright:
© 2025 The Royal Society of Chemistry.
PY - 2025/1/20
Y1 - 2025/1/20
N2 - Electrochemiluminescence (ECL) of luminol and electrocatalysis by Prussian blue were compared for the selective detection of H2O2 at the boron-doped diamond (BDD) electrodes. The H2O2 detection was optimized by various parameters such as the applied potential at pH 7.4, which is a physiological value usually used for H2O2 detection in enzymatic reactions. At an optimum applied potential of +0.5 V, a linear increase in the ECL signals (R2 = 0.99) was achieved for H2O2 concentrations ranging between 0 to 100 μM with an estimated limit of detection (LOD) of 2.59 μM. This LOD was better than that obtained with electrocatalysis measurements using the same electrode modified with Prussian blue. Furthermore, the interference study in the presence of glucose, Fe3+, Cl−, Ca2+, CO32−, Na+, and F− ions showed a comparable selectivity of the luminol ECL and PB-BDD electrochemical current. Nevertheless, the ECL method exhibited significant advantage in the high stability of its signal response.
AB - Electrochemiluminescence (ECL) of luminol and electrocatalysis by Prussian blue were compared for the selective detection of H2O2 at the boron-doped diamond (BDD) electrodes. The H2O2 detection was optimized by various parameters such as the applied potential at pH 7.4, which is a physiological value usually used for H2O2 detection in enzymatic reactions. At an optimum applied potential of +0.5 V, a linear increase in the ECL signals (R2 = 0.99) was achieved for H2O2 concentrations ranging between 0 to 100 μM with an estimated limit of detection (LOD) of 2.59 μM. This LOD was better than that obtained with electrocatalysis measurements using the same electrode modified with Prussian blue. Furthermore, the interference study in the presence of glucose, Fe3+, Cl−, Ca2+, CO32−, Na+, and F− ions showed a comparable selectivity of the luminol ECL and PB-BDD electrochemical current. Nevertheless, the ECL method exhibited significant advantage in the high stability of its signal response.
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U2 - 10.1039/d4ay02175d
DO - 10.1039/d4ay02175d
M3 - Article
AN - SCOPUS:85216620024
SN - 1759-9660
VL - 17
SP - 1790
EP - 1796
JO - Analytical Methods
JF - Analytical Methods
IS - 8
ER -