Comparison of clonogenic survival data obtained by pre-and post-irradiation methods

Takahiro Oike, Yuka Hirota, Narisa Dewi Maulany Darwis, Atsushi Shibata, Tatsuya Ohno

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF2, SF4, SF6, SF8, D10, or D50 (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.

Original languageEnglish
Article number171
Pages (from-to)1-6
Number of pages6
JournalJournal of Personalized Medicine
Volume10
Issue number4
DOIs
Publication statusPublished - 2020 Dec
Externally publishedYes

Keywords

  • Cancer
  • Clonogenic assays
  • Methods
  • Plating
  • Radiation
  • Radiosensitivity

ASJC Scopus subject areas

  • Medicine (miscellaneous)

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