Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins

Katsumi Kasashima, Eiji Sakashita, Kuniaki Saito, Hiroshi Sakamoto

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)


Hu proteins are RNA-binding proteins that are the vertebrate homologs of Drosophila ELAV, and are implicated in stabilization or enhanced translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Here, using the yeast two-hybrid system, we show that neuron-specific Hu proteins can interact with themselves. Immuno-precipitation assays demonstrated that the interaction between Hu proteins occurs in mammalian cells and is strongly enhanced in the presence of cellular RNA. Furthermore, using in situ chemical crosslinking assays, we found that HuD, one of the neuron-specific Hu proteins, multimerizes in cells. The crosslinked HuD multimers retain specific RNA-binding ability and can interact with additional Hu proteins. Consistent with this biochemical property, HuD showed granular distribution in two neurogenic cell lines. These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs.

Original languageEnglish
Pages (from-to)4519-4526
Number of pages8
JournalNucleic acids research
Issue number20
Publication statusPublished - 2002 Oct 15
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


Dive into the research topics of 'Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins'. Together they form a unique fingerprint.

Cite this