@article{7c9563f8659c4a0f8016ee06effb134f,
title = "Comprehensive metabolome analysis of intracellular metabolites in cultured cells",
abstract = "Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types.",
keywords = "Cell Biology, Cell culture, Mass Spectrometry, Metabolism, Metabolomics",
author = "Ryuhei Kudo and Kaori Igarashi and Tomoyoshi Soga and Takamasa Ishikawa and Yasuhiro Saito",
note = "Funding Information: We thank Dr. Masaru Tomita for supporting us on this project. This work is supported by funding from the Shonai Industrial Promotion Center (T.I. and Y.S.) and research funds from the Yamagata Prefectural Government and the City of Tsuruoka. The research of Y.S. is supported by a Grant-in-Aid for Scientific Research (C) JSPS KAKENHI grant number 20K07620, The Naito Foundation , The Princess Takamatsu Cancer Research Fund , The Kobayashi Foundation for Cancer Research , The Takeda Science Foundation , and The Sumitomo Foundation . Funding Information: We thank Dr. Masaru Tomita for supporting us on this project. This work is supported by funding from the Shonai Industrial Promotion Center (T.I. and Y.S.) and research funds from the Yamagata Prefectural Government and the City of Tsuruoka. The research of Y.S. is supported by a Grant-in-Aid for Scientific Research (C) JSPS KAKENHI grant number 20K07620, The Naito Foundation, The Princess Takamatsu Cancer Research Fund, The Kobayashi Foundation for Cancer Research, The Takeda Science Foundation, and The Sumitomo Foundation. R.K. K.I. T.S. T.I. and Y.S. designed and interpreted experiments and cowrote the paper. R.K. and T.I. performed CE-TOFMS and LC-QTOFMS analyses. R.K. is an employee of Infinity lab Co. Ltd. and T.I. is a president of the Infinity lab Co. Ltd. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = sep,
day = "16",
doi = "10.1016/j.xpro.2022.101531",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",
}
