Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

Michal Jaroš, Tomoyoshi Soga, Tom van de Goor, Bohuslav Gaš

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)


A simple rule stating that the signal in conductivity detection in capillary zone electrophoresis is proportional to the difference between the analyte mobility and mobility of the background electrolyte (BGE) co-ion is valid only for systems with fully ionized electrolytes. In zone electrophoresis systems with weak electrolytes both conductivity signal and electromigration dispersion of analyte peaks depend on the conductivity and pH effects. This allows optimization of the composition of BGEs to give a good conductivity signal of analytes while still keeping electromigration dispersion near zero, regardless of the injected amount of sample. The demands to achieve minimum electromigration dispersion and high sensitivity in conductivity detection can be accomplished at the same time. PeakMaster software is used for inspection of BGEs commonly used for separation of sugars (carbohydrates, saccharides) at highly alkaline pH. It is shown that the terms direct and indirect conductivity detection are misleading and should not be used.

Original languageEnglish
Pages (from-to)1948-1953
Number of pages6
Issue number10
Publication statusPublished - 2005 May


  • Background electrolyte
  • Capillary electrophoresis
  • Conductivity detection
  • Electromigration dispersion
  • PeakMaster software

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry


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