Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes

K. Inoue; R. Kawano; H. Yasuga; M. Takinoue; T. Osaki; K. Kamiya; N. Miki; S. Takeuchi

doi:10.1109/TRANSDUCERS.2015.7181029

Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes

K. Inoue, R. Kawano, H. Yasuga, M. Takinoue, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This paper experimentally demonstrated the connection of logic gates using DNA and membrane protein's channel reconstituted into bilayer lipid membranes (BLMs). In this system, droplets worked as the carrier of DNA to convey it as biological signal to next stage and reformation and rupture of BLM accelerated DNA transfer between droplets. Furthermore, DNA is detected based on the electrical and physical characters of DNA translocation via membrane protein's channel. In this study, we successfully demonstrated NOR operation by connecting OR and NOT gate. This system is readily applicable to develop high sensitive biosensor and computing system imitating biological information processing.

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	739-742
Number of pages	4
ISBN (Electronic)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181029
Publication status	Published - 2015 Aug 5
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: 2015 Jun 21 → 2015 Jun 25

Publication series

Name	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	15/6/21 → 15/6/25

Keywords

DNA computing
Logic gate
Membrane protein
Planar lipid bilayer
Single molecule detection
hybridization

ASJC Scopus subject areas

Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2015.7181029

Cite this

Inoue, K., Kawano, R., Yasuga, H., Takinoue, M., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2015). Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 739-742). Article 7181029 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181029

Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. / Inoue, K.; Kawano, R.; Yasuga, H. et al.
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 739-742 7181029 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Inoue, K, Kawano, R, Yasuga, H, Takinoue, M, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2015, Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181029, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 739-742, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 15/6/21. https://doi.org/10.1109/TRANSDUCERS.2015.7181029

Inoue K, Kawano R, Yasuga H, Takinoue M, Osaki T, Kamiya K et al. Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 739-742. 7181029. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). doi: 10.1109/TRANSDUCERS.2015.7181029

Inoue, K. ; Kawano, R. ; Yasuga, H. et al. / Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 739-742 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

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AU - Osaki, T.

AU - Kamiya, K.

AU - Miki, N.

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AB - This paper experimentally demonstrated the connection of logic gates using DNA and membrane protein's channel reconstituted into bilayer lipid membranes (BLMs). In this system, droplets worked as the carrier of DNA to convey it as biological signal to next stage and reformation and rupture of BLM accelerated DNA transfer between droplets. Furthermore, DNA is detected based on the electrical and physical characters of DNA translocation via membrane protein's channel. In this study, we successfully demonstrated NOR operation by connecting OR and NOT gate. This system is readily applicable to develop high sensitive biosensor and computing system imitating biological information processing.

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Connectable DNA-logic operation using droplets and rupture/reformation of bilayer lipid membranes

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